anti-UPF3A antibody

Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2- UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation. Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.) Uniprot: ID Q9H1J1 Immunogen: UPF3 regulator of nonsense transcripts homolog A (yeast) Recommended dilution: WB : 1:500-1:5000;IHC：1:20-1:200；IF:1：20-1：200 Host: RabbitApplications Tested/Suitable: ELISA,WB,IHC