Endo H (Endoglycosidase H) – Specific Enzyme for High-Mannose N-Glycan Removal

Endo H (Endoglycosidase H) is a highly specific glycosidase enzyme widely used in glycobiology, protein research, and structural biology. It is designed for the selective removal of high-mannose and certain hybrid N-linked glycans from glycoproteins, enabling researchers to analyze protein structure and function with precision.

 

How Endo H Works

Endo H cleaves the bond between the two N-acetylglucosamine (GlcNAc) residues in the glycan core of glycoproteins. After cleavage, one GlcNAc remains attached to the asparagine residue, allowing for controlled and predictable glycan removal. This makes it an essential tool for:

• Studying protein glycosylation patterns
• Monitoring protein folding and quality control
• Conducting structural biology and crystallography experiments

Key Features

• High specificity: Targets high-mannose and certain hybrid N-linked glycans only.
• Reliable performance: Ideal for in vitro glycoprotein analysis.
• Essential for research: Supports glycoproteomics, structural studies, and enzyme function experiments.

Applications

Endo H is widely used in biochemistry and molecular biology laboratories for:

• Glycan characterization and profiling
• Protein engineering and expression studies
• Investigating post-translational modifications