OxiSelect™ Protein Carbonyl ELISA Assay – 96-Well Plate
The OxiSelect™ Protein Carbonyl ELISA Assay (96-well plate) is a sensitive and convenient immunoassay designed for the quantitative measurement of protein carbonyls, one of the most widely used biomarkers of protein oxidation and oxidative stress. This ready-to-use kit is ideal for basic research, preclinical studies, and oxidative stress profiling in a wide range of biological samples.
Principle of the Assay
Protein carbonyls formed during oxidative damage are first derivatized with dinitrophenylhydrazine (DNPH) to create stable DNP-protein adducts. These adducts are then captured in a 96-well plate and detected using a DNP-specific antibody, followed by a colorimetric readout. The absorbance is proportional to the carbonyl content in the sample.
Key Features
- Quantitative detection of protein carbonyls – reliable marker of protein oxidation and oxidative stress
- 96-well ELISA format – supports medium- to high-throughput screening
- High sensitivity and reproducibility – suitable for low-level oxidative damage detection
- Compatible with multiple sample types – cells, tissues, plasma, serum, and other biological fluids
- User-friendly protocol – standard ELISA workflow with clear, colorimetric endpoint
Applications
The OxiSelect™ Protein Carbonyl ELISA Assay is ideal for:
- Assessing oxidative stress in disease models (neurodegeneration, cardiovascular disease, diabetes, aging, etc.)
- Evaluating oxidative damage in cell culture and tissue samples
- Studying redox biology, antioxidant efficacy, and drug screening
- Monitoring protein oxidation in toxicology and environmental exposure studies
Format
- Plate size: 96-well microplate
- Assay type: Colorimetric ELISA
- Typical use: Up to 96 tests, depending on standards and replicates