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PNGase F (100 U/μL) – High-Activity N-Glycan Removal Enzyme

PNGase F (Peptide-N-Glycosidase F, EC 3.5.1.52) is a recombinant glycosidase designed for the precise and efficient removal of N-linked glycans from glycoproteins. Supplied at 100 U/μL, this concentrated enzyme provides high activity and consistency for analytical, structural, and biopharmaceutical workflows.

Key Features

  • High Activity: Supplied at 100 U/μL for precise dosing and cost-effective experiments.
  • Broad Specificity: Removes high-mannose, hybrid, and complex N-glycans from both denatured and native proteins, depending on glycosylation site accessibility.
  • Recombinant, Animal-Free: Ensures batch-to-batch consistency and eliminates animal-derived contaminants.
  • Mild Reaction Conditions: Active at pH 7.5–8.6, 37 °C, preserving protein integrity during deglycosylation.
  • Flexible Formulation: Supplied in a stabilizing buffer; glycerol-free variants available for sensitive downstream applications.

Specifications

  • Enzyme Class: EC 3.5.1.52 (Peptide-N-Glycosidase F)
  • Concentration: 100 U/μL (defined by glycan release from a standard glycoprotein substrate)
  • Molecular Weight: ~34 kDa
  • Storage: −20 °C; avoid repeated freeze–thaw cycles

Applications

PNGase F (100 U/μL) is ideal for:

  • Deglycosylation of glycoproteins for analytical characterization
  • Structural biology studies including crystallography and NMR
  • Biopharmaceutical quality control for therapeutic glycoproteins
  • Glycan profiling and mass spectrometry analysis

Advantages

The high concentration and specificity of PNGase F (100 U/μL) make it a reliable and efficient tool for laboratories requiring consistent, reproducible N-glycan removal, enabling streamlined workflows and accurate downstream analysis.